オカザキ　ケン   KEN OKAZAKI   岡崎　賢    所属   医学部 医学科（東京女子医科大学病院）    職種   教授・基幹分野長
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Characterisation of osteophytes as an autologous bone graft source: An experimental study in vivo and in vitro.
掲載誌名	正式名：Bone & joint research 略　 称：Bone Joint Res ISSNコード：20463758/20463758
掲載区分	国外
巻・号・頁	6(2),pp.73-81
著者・共著者	Ishihara K, †, Okazaki K*, Akiyama T, Akasaki Y, Nakashima Y
担当区分	2nd著者,責任著者
発行年月	2017/02
概要	OBJECTIVES:Osteophytes are products of active endochondral and intramembranous ossification, and therefore could theoretically provide significant efficacy as bone grafts. In this study, we compared the bone mineralisation effectiveness of osteophytes and cancellous bone, including their effects on secretion of growth factors and anabolic effects on osteoblasts.METHODS:Osteophytes and cancellous bone obtained from human patients were transplanted onto the calvaria of severe combined immunodeficient mice, with Calcein administered intra-peritoneally for fluorescent labelling of bone mineralisation. Conditioned media were prepared using osteophytes and cancellous bone, and growth factor concentration and effects of each graft on proliferation, differentiation and migration of osteoblastic cells were assessed using enzyme-linked immunosorbent assays, MTS ((3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)) assays, quantitative real-time polymerase chain reaction, and migration assays.RESULTS:After six weeks, the area of mineralisation was significantly higher for the transplanted osteophytes than for the cancellous bone (43803 μm2, sd 14660 versus 9421 μm2, sd 5032, p = 0.0184, one-way analysis of variance).
DOI	10.1302/2046-3758.62.BJR-2016-0199.R1
PMID	28148490