KOBAYASHI Hirohito    Department   School of Medicine(Tokyo Women's Medical University Adachi Medical Center), School of Medicine    Position   Associate Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Expression and function of PD-1 in human γδ T cells that recognize phosphoantigens.
Journal	Formal name：European journal of immunology Abbreviation：Eur J Immunol ISSN code：15214141/00142980
Domestic / Foregin	Foregin
Volume, Issue, Page	41(2),pp.345-355
Author and coauthor	Iwasaki Masashi, Tanaka Yoshimasa, Kobayashi Hirohito, Murata-Hirai Kaoru, Miyabe Hideto, Sugie Tomoharu, Toi Masakazu, Minato Nagahiro
Publication date	2011/02
Summary	Programmed cell death-1 (PD-1) is an inhibitory receptor and plays an important role in the regulation of αβ T cells. Little is known, however, about the role of PD-1 in γδ T cells. In this study, we investigated the expression and function of PD-1 in human γδ T cells. Expression of PD-1 was rapidly induced in primary γδ T cells following antigenic stimulation, and the PD-1(+) γδ T cells produced IL-2. When PD-1(+) γδ T cells were stimulated with Daudi cells with and without programmed cell death ligand-1 (PD-L1) expression, the levels of IFN-γ production and cytotoxicity in response to PD-L1(+) Daudi cells were diminished compared to the levels seen in response to PD-L1(-) Daudi cells. The attenuated effector functions were reversed by anti-PD-L1 mAb. When PD-1(+) γδ T cells were challenged by PD-L1(+) tumors pretreated with zoledronate (Zol), which induced γδ TCR-mediated signaling, the resulting reduction in cytokine production was only slight to moderate compared to the reduction seen when PD-1(+) γδ T cells were challenged by PD-L1(-) tumors. In addition, cytotoxic activity of PD-1(+) γδ T cells against Zol-treated PD-L1(+) tumors was comparable to that against Zol-treated PD-L1(-) tumors. These results suggest that TCR triggering may partially overcome the inhibitory effect of PD-1 in γδ T cells.
DOI	10.1002/eji.201040959
PMID	21268005