AKIMASA ICHINOE
   Department   School of Medicine(Tokyo Women's Medical University Adachi Medical Center), School of Medicine
   Position   Assistant Professor
Article types Original article
Language English
Peer review Peer reviewed
Title Identification of the Critical Site of Calponin 1 for Suppression of Ovarian Cancer Properties.
Journal Formal name:Anticancer research
Abbreviation:Anticancer Res
ISSN code:02507005/17917530
Volume, Issue, Page 35(11),pp.5996-5999
Author and coauthor TAKAKO YAMANE†, KAZUO ASANOMA, HIROAKI KOBAYASHI, GE LIU, HIROSHI YAGI, TATSUHIRO OHGAMI, AKIMASA ICHINOE, KENZO SONODA, NORIO WAKE, KIYOKO KATO
Publication date 2015/11
Summary Background: Although several studies have
demonstrated the tumor suppressive function of CNN1
(calponin 1), no studies have performed a site-specific
analysis of CNN1 on tumor cell activities. Materials and
Methods: We herein studied the site-specific effects of CNN1
in ovarian cancer cells using full-length CNN1 (fCNN1),
three CNN1 repeats (3CNRs), or the first CNN1 repeat
(CNR1) expression vectors. Ovarian cancer cells stably
expressing each construct were analyzed for in vitro
proliferation, cell motility, invasion, and soft agar assays.
An in vitro model of pleural dissemination was also
established. Results: Cell proliferation, anchorageindependent
colony formation, cell motility, and cell
invasion were all suppressed in fCNN1, 3CNRs, and CNR1-
stably-expressing cells. CNN1 expression in mesothelial
cells suppressed cancer cell invasion into a monolayer of
mesothelial cells. Conclusion: CNR1 showed similar
suppressive effects as fCNN1. Results suggest CNR1 as a
potential small synthetic peptide candidate for therapeutic
strategies against ovarian cancer.