AKIMASA ICHINOE    Department   School of Medicine(Tokyo Women's Medical University Adachi Medical Center), School of Medicine    Position   Assistant Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Identification and characterization of two forms of mouse MUTYH proteins encoded by alternatively spliced transcripts.
Journal	Formal name：Nucleic acids research Abbreviation：Nucleic Acids Res ISSN code：03051048/13624962
Domestic / Foregin	Foregin
Volume, Issue, Page	32(2),pp.477-487
Total page number	11
Author and coauthor	Akimasa Ichinoe†, Behmanesh Mehrdad, Yohei Tominaga, Yasuhiro Ushijima, Seiki Hirano, Yasunari Sakai, Daisuke Tsuchimoto, Kunihiko Sakumi, Norio Wake, Yusaku Nakabeppu
Authorship	Lead author
Publication date	2004/01/23
Summary	There are three types of mouse Mutyh mRNAs (type a, b and c) generated by alternative splicing, and type b mRNA is a major form among the three in most of the tissues examined. The level of type c mRNA is relatively high in brain. Type a and b mRNAs were expected to encode 57.7 kDa protein (MUTYHalpha), while type c mRNA had a partly different open reading frame encoding a 50.2 kDa protein (MUTYHbeta). An in vitro translation of type b and c mRNAs produced a 50 kDa MUTYHalpha and 47 kDa MUTYHbeta, respectively. MUTYHalpha and MUTYHbeta were detected in wild-type embryonic stem (ES) cells or thymocytes prepared from wild-type mice, but neither MUTYH-null ES cells nor thymocytes prepared from MUTYH-null mice. Both MUTYHalpha and MUTYHbeta were mainly localized in the nuclei and some in mitochondria in wild-type ES cells. Recombinant MUTYHalpha and beta were expressed as fusion proteins with thioredoxin in Escherichia coli, but only MUTYHalpha was partly soluble and thus could be purified. Recombinant MUTYHalpha possessed DNA glycosylase activities to excise adenine opposite 8-oxoguanine and guanine but not AP lyase activity.
DOI	10.1093/nar/gkh214