Haraguchi Yuji    Department   Research Institutes and Facilities, Research Institutes and Facilities    Position   Associate Professor (Fixed Term)
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Proliferation and differentiation of primary bovine myoblasts using Chlorella vulgaris extract for sustainable production of cultured meat.
Journal	Formal name：Biotechnology progress Abbreviation：Biotechnol Prog ISSN code：15206033/15206033
Domestic / Foregin	Foregin
Volume, Issue, Page	38(3),pp.e3239
Author and coauthor	OKAMOTO Yuta†, HARAGUCHI Yuji*, YOSHIDA Azumi, TAKAHASHI Hironobu, YAMANAKA Kumiko, SAWAMURA Naoya, ASAHI Toru, SHIMIZU Tatsuya*
Authorship	2nd author,Corresponding author
Publication date	2022/03/30
Summary	Recently, cultured meat obtained from livestock-derived cells is being considered as a sustainable food source that reduces the use of natural resources. This study aimed to show that nutrients extracted from Chlorella vulgaris were beneficial in the culture of primary bovine myoblasts (PBMs), a major cell source for cultured meat production. Nutrients (glucose, amino acids, and vitamins) present in the animal-cell culture media were effectively recovered from C. vulgaris using acid hydrolysis treatment. On culture in nutrient-free inorganic salt solution (ISS), cell death was induced in most PBMs after 6 days of cultivation. However, the addition of C. vulgaris extract (CVE) significantly improved PBM viability, which was comparable to the viability in conventional culture medium (Dulbecco's modified Eagle's medium [DMEM]). Furthermore, by adding horse serum to induce differentiation, the formation of myotubes was confirmed when CVE were used. Together, the results showed that CVE could be used as an alternative to the conventional culture medium for PBMs. These findings will not only lower the environmental risks associated with the establishment of this eco-friendly cell culture system, but also highlight microalgae as a potent nutrient source that can replace conventional grain-dependent nutrient sources. This article is protected by copyright. All rights reserved.
DOI	10.1002/btpr.3239
PMID	35073462