Haraguchi Yuji
   Department   Research Institutes and Facilities, Research Institutes and Facilities
   Position   Associate Professor (Fixed Term)
Article types Original article
Language English
Peer review Peer reviewed
Title Rapid creation system of morphologically and functionally communicative three-dimensional cell-dense tissue by centrifugation.
Journal Formal name:Biotechnology progress
Abbreviation:Biotechnol Prog
ISSN code:(1520-6033)1520-6033(Linking)
Domestic / ForeginForegin
Publisher Wiley
Volume, Issue, Page 34(6),pp.1447-1453
Author and coauthor HARAGUCHI Yuji†, MATSUURA Katsuhisa, KAGAWA Yuki, HASEGAWA Akiyuki, KUBO Hirotsugu, SHIMIZU Tatsuya*
Authorship Lead author
Publication date 2018/12
Summary This study reports a rapid fabrication system of a morphologically and functionally communicative 3-D cell-dense tissue without scaffolds by centrifugation. The tight adhesion between C2C12 myoblasts and culture surface was accelerated without significant cell damage by centrifugation (80 x g, 37°C, 30 min). A thicker tissue created on a temperature-responsive culture surface was harvested by decreasing temperature. The 3D myoblast tissues having approximately 200 µm-thickness were created at 1.5 h [centrifugation (80 x g, 37°C) for 30 min and tissue harvest for 1 h]. However, in the case of without centrifugation, the myoblast tissues had fragile parts even at 7.5 h after the incubation. Additionally, electrically/functionally communicative and thicker human induced pluripotent stem (iPS) cell-derived cardiac tissues were created rapidly by the centrifugation and cultivation at 37°C. We report a centrifugation system that significantly shortens the creation time of 3D tissues. We envision that this procedure will contribute to the field of tissue engineering and regenerative medicine. This article is protected by copyright. All rights reserved.
DOI 10.1002/btpr.2691
PMID 30009512