ハラグチ ユウジ   Haraguchi Yuji
  原口 裕次
   所属   研究施設 研究施設
   職種   特任講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Rapid creation system of morphologically and functionally communicative three-dimensional cell-dense tissue by centrifugation.
掲載誌名 正式名:Biotechnology progress
略  称:Biotechnol Prog
ISSNコード:(1520-6033)1520-6033(Linking)
掲載区分国外
出版社 Wiley
巻・号・頁 34(6),1447-1453頁
著者・共著者 HARAGUCHI Yuji†, MATSUURA Katsuhisa, KAGAWA Yuki, HASEGAWA Akiyuki, KUBO Hirotsugu, SHIMIZU Tatsuya*
発行年月 2018/12
概要 This study reports a rapid fabrication system of a morphologically and functionally communicative 3-D cell-dense tissue without scaffolds by centrifugation. The tight adhesion between C2C12 myoblasts and culture surface was accelerated without significant cell damage by centrifugation (80 x g, 37°C, 30 min). A thicker tissue created on a temperature-responsive culture surface was harvested by decreasing temperature. The 3D myoblast tissues having approximately 200 µm-thickness were created at 1.5 h [centrifugation (80 x g, 37°C) for 30 min and tissue harvest for 1 h]. However, in the case of without centrifugation, the myoblast tissues had fragile parts even at 7.5 h after the incubation. Additionally, electrically/functionally communicative and thicker human induced pluripotent stem (iPS) cell-derived cardiac tissues were created rapidly by the centrifugation and cultivation at 37°C. We report a centrifugation system that significantly shortens the creation time of 3D tissues. We envision that this procedure will contribute to the field of tissue engineering and regenerative medicine. This article is protected by copyright. All rights reserved.
DOI 10.1002/btpr.2691
PMID 30009512