Haraguchi Yuji
   Department   Research Institutes and Facilities, Research Institutes and Facilities
   Position   Assistant Professor (Fixed Term)
Article types Other
Language English
Peer review Peer reviewed
Title Massively-multicellular alignment with the self-aggregate of air bubbles.
Journal Formal name:Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
Abbreviation:Conf Proc IEEE Eng Med Biol Soc
ISSN code:(1557-170X)1557-170X(Linking)
Domestic / ForeginForegin
Volume, Issue, Page 2015,3537-40頁
Author and coauthor Tanaka Nobuyuki†, Haraguchi Yuji, Shimizu Tatsuya, Yamato Masayuki, Okano Teruo, Miyake Jun
Publication date 2015/08
Summary This study proposes a cell manipulation method with aggregated air bubbles on cell culture medium. This method requires no additional regents nor devices, except for normal cell-culture materials such as cell culture dishes and pipettes. Bubbles generated by pipetting were spontaneously aggregated with regularity on the whole surface and used as a mask for avoiding cell adhesion after cell-seeding. The diameter of bubbles was able to be controlled by the size of micro-pipette tips. Seeded cells spread to the whole area along the bubble gap. This technique is a surface-tension-driven self-assembly-based method. Using this technique, millions of living cells were successfully aligned into a hexagonal pattern within 300 μm in pattern width on the whole surface of dish for less than 2 h.
DOI 10.1109/EMBC.2015.7319156
PMID 26737056