Haraguchi Yuji
   Department   Research Institutes and Facilities, Research Institutes and Facilities
   Position   Associate Professor (Fixed Term)
Article types Original article
Language English
Peer review Peer reviewed
Title New isolation system for collecting living cells from tissue
Journal Formal name:Journal of Bioscience and Bioengineering
Abbreviation:J Biosci Bioeng
ISSN code:13474421(Electronic)13474421(Linking)
Volume, Issue, Page 115(1),pp.100-103
Author and coauthor SHIOYAMA Takahiro†, HARAGUCHI Yuji, MURAGAKI Yoshihiro, SHIMIZU Tatsuya, OKANO Teruo
Publication date 2013/01
Summary A new semi-automatic living-cell isolation system was developed. The new system improves the quality of isolated cells, reduces cell isolation time, and isolates more cells with a higher cell viability compared to conventional manual methods. We successfully applied this system to isolate beating cardiomyocytes and fabricate electrical communicative cardiac tissue. In this study using the isolated cardiac cells we also fabricated a cardiac cell sheet that beat spontaneously and synchronously.
DOI 10.1016/j.jbiosc.2012.08.013
Document No. 23063341