タケハラ サチコ
  竹原 祥子
   所属   医学部 医学科
   職種   助教
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Characterization of oral microbiota in marmosets: Feasibility of using the marmoset as a human oral disease model.
掲載誌名 正式名:PloS one
略  称:PLoS One
ISSNコード:(1932-6203)1932-6203(Linking)
掲載区分国外
巻・号・頁 14(2),e0207560頁
著者・共著者 Takehara Sachiko*†, Zeredo Jorge L, Kumei Yasuhiro, Kagiyama Kensuke, Fukasawa Kazumasa, Oshiro Akiko, Ueno Masayuki, Kojimahara Noriko, Minakuchi Shunsuke, Kawaguchi Yoko
発行年月 2019/02
概要 With rapid aging of the world's population, the demand for research, for a better understanding of aging and aging-related disorders, is increasing. Ideally, such research should be conducted on human subjects. However, due to ethical considerations, animals such as rodents and monkeys are used as alternatives. Among these alternative models, non-human primates are preferred because of their similarities with humans. The small South American common marmoset (Callithrix jacchus) may offer several advantages over other non-human primates in terms of its smaller size, shorter life-span, and dental anatomy identical to humans. The purpose of this study was to determine the viability of using the marmoset as a human oral disease model. We collected saliva samples from eight marmosets and eight human subjects. Prokaryotic DNA was extracted from the saliva samples, and 16S bacterial rRNA gene sequencing was performed on each of the samples. Our results indicated that the types of oral microbiomes detected among human and marmoset samples were nearly indistinguishable. In contrast, the oral microbiomes of our human and marmoset subjects were distinctly different from those reported for rats and dogs, which are currently popular research animals. The oral microbiomes of marmosets showed greater diversity than those of humans. However, the oral microbiota of marmosets exhibited less variation than those of humans, which may be attributed to the fact that the marmoset subjects were kept in a controlled environment with identical lifestyles. The characteristics of its oral microbiota, combined with other technical advantages, suggest that the marmoset may provide the best animal model thus far for the study of oral health. This study characterized the oral microbes of the marmoset, thereby providing information to support future application of the marmoset as a model for age-related oral disease.
DOI 10.1371/journal.pone.0207560
PMID 30730898