Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine
   Position   Assistant Professor
Article types Original article
Language English
Peer review Peer reviewed
Title Short consensus probes with 3'-minor groove binder of the immunoglobulin heavy-chain gene for real-time quantitative PCR in B-cell non-Hodgkin lymphomas.
Journal Formal name:Laboratory investigation; a journal of technical methods and pathology
Abbreviation:Lab Invest
ISSN code:00236837/00236837
Domestic / ForeginForegin
Volume, Issue, Page 84(7),pp.932-936
Author and coauthor Uchiyama Michihiro, Maesawa Chihaya, Yashima-Abo Akiko, Tarusawa Mitsu, Satoh Mamoru, Satoh Takashi, Ishida Yoji, Ito Shigeki, Murai Kazunori, Enomoto Sanae, Utsugisawa Taiju, Masuda Tomoyuki
Publication date 2004/07
Summary We used 3'-minor groove binder (MGB) technology to develop consensus fluorogenically labeled probes of the immunoglobulin heavy-chain (IgH) gene for detecting minimal residual disease (MRD) in B-cell non-Hodgkin lymphoma (B-NHL). Sequence data from 59 patients with B-NHLs revealed a narrow consensus region as a result of somatic hypermutations and variable VH usage, indicating that it would be difficult to design ordinary non-MGB probes. MGB probes, characterized by shorter length but higher melting temperature, are more suitable for this situation than ordinary non-MGB probes. In fact, the present data indicated that about 20% more cases were detectable with MGB probes (34/59, 57.6%) than with the non-MGB probes (23/59, 39.0%) designed by Donovan et al. MGB technology is useful for the design of consensus fluorogenically labeled probes of the IgH gene for detecting MRD.
DOI 10.1038/labinvest.3700092
PMID 15034598