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UTSUGISAWA Taiju
Department School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine Position Associate Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Short consensus probes with 3'-minor groove binder of the immunoglobulin heavy-chain gene for real-time quantitative PCR in B-cell non-Hodgkin lymphomas. |
| Journal | Formal name：Laboratory investigation; a journal of technical methods and pathology Abbreviation：Lab Invest ISSN code：00236837/00236837 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 84(7),pp.932-936 |
| Author and coauthor | Uchiyama Michihiro, Maesawa Chihaya, Yashima-Abo Akiko, Tarusawa Mitsu, Satoh Mamoru, Satoh Takashi, Ishida Yoji, Ito Shigeki, Murai Kazunori, Enomoto Sanae, Utsugisawa Taiju, Masuda Tomoyuki |
| Publication date | 2004/07 |
| Summary | We used 3'-minor groove binder (MGB) technology to develop consensus fluorogenically labeled probes of the immunoglobulin heavy-chain (IgH) gene for detecting minimal residual disease (MRD) in B-cell non-Hodgkin lymphoma (B-NHL). Sequence data from 59 patients with B-NHLs revealed a narrow consensus region as a result of somatic hypermutations and variable VH usage, indicating that it would be difficult to design ordinary non-MGB probes. MGB probes, characterized by shorter length but higher melting temperature, are more suitable for this situation than ordinary non-MGB probes. In fact, the present data indicated that about 20% more cases were detectable with MGB probes (34/59, 57.6%) than with the non-MGB probes (23/59, 39.0%) designed by Donovan et al. MGB technology is useful for the design of consensus fluorogenically labeled probes of the IgH gene for detecting MRD. |
| DOI | 10.1038/labinvest.3700092 |
| PMID | 15034598 |