ウツギサワ タイジユ   UTSUGISAWA Taiju
  槍澤 大樹
   所属   医学部 医学科(東京女子医科大学病院)
   職種   講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Short consensus probes with 3'-minor groove binder of the immunoglobulin heavy-chain gene for real-time quantitative PCR in B-cell non-Hodgkin lymphomas.
掲載誌名 正式名:Laboratory investigation; a journal of technical methods and pathology
略  称:Lab Invest
ISSNコード:00236837/00236837
掲載区分国外
巻・号・頁 84(7),pp.932-936
著者・共著者 Uchiyama Michihiro, Maesawa Chihaya, Yashima-Abo Akiko, Tarusawa Mitsu, Satoh Mamoru, Satoh Takashi, Ishida Yoji, Ito Shigeki, Murai Kazunori, Enomoto Sanae, Utsugisawa Taiju, Masuda Tomoyuki
発行年月 2004/07
概要 We used 3'-minor groove binder (MGB) technology to develop consensus fluorogenically labeled probes of the immunoglobulin heavy-chain (IgH) gene for detecting minimal residual disease (MRD) in B-cell non-Hodgkin lymphoma (B-NHL). Sequence data from 59 patients with B-NHLs revealed a narrow consensus region as a result of somatic hypermutations and variable VH usage, indicating that it would be difficult to design ordinary non-MGB probes. MGB probes, characterized by shorter length but higher melting temperature, are more suitable for this situation than ordinary non-MGB probes. In fact, the present data indicated that about 20% more cases were detectable with MGB probes (34/59, 57.6%) than with the non-MGB probes (23/59, 39.0%) designed by Donovan et al. MGB technology is useful for the design of consensus fluorogenically labeled probes of the IgH gene for detecting MRD.
DOI 10.1038/labinvest.3700092
PMID 15034598