Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine
   Position   Assistant Professor
Article types Original article
Language English
Peer review Peer reviewed
Title Quantitative assessment of contaminating tumor cells in autologous peripheral blood stem cells of B-cell non-Hodgkin lymphomas using immunoglobulin heavy chain gene allele-specific oligonucleotide real-time quantitative-polymerase chain reaction.
Journal Formal name:Leukemia research
Abbreviation:Leuk Res
ISSN code:01452126/01452126
Domestic / ForeginForegin
Volume, Issue, Page 27(10),pp.925-934
Author and coauthor Yashima Akiko, Maesawa Chihaya, Uchiyama Michihiro, Tarusawa Mitsu, Satoh Takashi, Satoh Mamiko, Enomoto Sanae, Sugawara Ken, Numaoka Hideharu, Murai Kazunori, Utsugisawa Taiju, Ishida Yoji, Masuda Tomoyuki
Publication date 2003/10
Summary A real-time quantitative-polymerase chain reaction (RQ-PCR) targeting the immunoglobulin heavy chain (IgH) gene has been used for the quantification of minimal residual disease (MRD) in B-cell hematological malignancies. In non-Hodgkin lymphoma (NHL), experimental costs are increased, as a large number of primer-probe sets are required because of diversity, due to somatic and ongoing mutations of the IgH gene. We developed an allele-specific oligonucleotide (ASO) combined with a germline consensus probe-based RQ-PCR assay and examined MRD in peripheral blood stem cells (PBSC). The IgH consensus probes were adapted in seven (50%) of 14 amplifiable cases. Patients with heavily contaminating tumor cells in PBSC relapsed after PBSC transplantation. Our strategy will contribute to the development of a cost-efficient, precisely quantitative and systemic detection assay for MRD in NHL.
DOI 10.1016/s0145-2126(03)00049-3
PMID 12860013