UTSUGISAWA Taiju    Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine    Position   Associate Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Quantitative assessment of contaminating tumor cells in autologous peripheral blood stem cells of B-cell non-Hodgkin lymphomas using immunoglobulin heavy chain gene allele-specific oligonucleotide real-time quantitative-polymerase chain reaction.
Journal	Formal name：Leukemia research Abbreviation：Leuk Res ISSN code：01452126/01452126
Domestic / Foregin	Foregin
Volume, Issue, Page	27(10),pp.925-934
Author and coauthor	Yashima Akiko, Maesawa Chihaya, Uchiyama Michihiro, Tarusawa Mitsu, Satoh Takashi, Satoh Mamiko, Enomoto Sanae, Sugawara Ken, Numaoka Hideharu, Murai Kazunori, Utsugisawa Taiju, Ishida Yoji, Masuda Tomoyuki
Publication date	2003/10
Summary	A real-time quantitative-polymerase chain reaction (RQ-PCR) targeting the immunoglobulin heavy chain (IgH) gene has been used for the quantification of minimal residual disease (MRD) in B-cell hematological malignancies. In non-Hodgkin lymphoma (NHL), experimental costs are increased, as a large number of primer-probe sets are required because of diversity, due to somatic and ongoing mutations of the IgH gene. We developed an allele-specific oligonucleotide (ASO) combined with a germline consensus probe-based RQ-PCR assay and examined MRD in peripheral blood stem cells (PBSC). The IgH consensus probes were adapted in seven (50%) of 14 amplifiable cases. Patients with heavily contaminating tumor cells in PBSC relapsed after PBSC transplantation. Our strategy will contribute to the development of a cost-efficient, precisely quantitative and systemic detection assay for MRD in NHL.
DOI	10.1016/s0145-2126(03)00049-3
PMID	12860013