上芝　秀博    Department   School of Medicine, School of Medicine    Position   Assistant Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Phylogenetic relationship of Pasteurella pneumotropica isolates from laboratory rodents based on 16S rDNA sequence.
Journal	Formal name：The Journal of veterinary medical science / the Japanese Society of Veterinary Science Abbreviation：J Vet Med Sci ISSN code：(0916-7250)0916-7250(Linking)
Volume, Issue, Page	68(6),pp.639-41
Author and coauthor	SASAKI Hiraku, KAWAMOTO Eiichi, UESHIBA Hidehiro, AMAO Hiromi, SAWADA Takuo
Publication date	2006/06
Summary	A 1344 bp fragment of the 16S ribosomal DNA (rDNA) sequence was used to determine the genetic relationship of Pasteurella pneumotropica isolates from laboratory rodents. A total of 30 nucleotide sequences of P. pneumotropica, including 24 wild strains, 3 reference strains, and 3 nucleotide sequences deposited in GenBank, were examined for heterogeneity of their 16S rDNA sequences. Phylogenetic analysis based on 16S rDNA sequence discriminated 5 types of branching lineages. Of these 5 types, 3 types had significant associations with mice or rats, and 2 had significant associations with the beta-hemolytic phenotype. These results suggest that 16S rDNA sequencing of P. pneumotropica isolates demonstrates genetic heterogeneity and phylogenetic discrimination in terms of their hemolytic phenotype and host associations.
PMID	16820726