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上芝 秀博
Department School of Medicine, School of Medicine Position Assistant Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus. |
| Journal | Formal name：Virology Abbreviation：Virology ISSN code：(1096-0341)0042-6822(Linking) |
| Volume, Issue, Page | 380(1),pp.99-108 |
| Author and coauthor | Hatakeyama Seisuke, Matsuoka Yusuke, Ueshiba Hidehiro, Komatsu Nobukazu, Itoh Kyogo, Shichijo Shigeki, Kanai Takao, Fukushi Masaya, Ishida Isao, Kirikae Teruo, Sasazuki Takehiko, Miyoshi-Akiyama Tohru |
| Publication date | 2008/10 |
| Summary | When expressed in mammalian cells, the nucleocapsid (N) and membrane (M) proteins of the severe acute respiratory syndrome coronavirus (SARS-CoV) are sufficient to form pseudoparticles. To identify region(s) of the N molecule required for pseudoparticle formation, we performed biochemical analysis of the interaction of N mutants and M in HEK293 cells. Using a peptide library derived from N, we found that amino acids 101-115 constituted a novel binding site for M. We examined the ability of N mutants to interact with M and form pseudoparticles, and our observations indicated that M bound to NDelta(101-115), N1-150, N151-300, and N301-422, but not to N1-150Delta(101-115). However, pseudoparticles were formed when NDelta(101-115) or N301-422, but not N1-150 or N151-300, were expressed with M in HEK293 cells. These results indicated that the minimum portion of N required for the interaction with M and pseudoparticle formation consists of amino acids 301-422. |
| DOI | 10.1016/j.virol.2008.07.012 |
| PMID | 18703211 |