ストウ　チカコ   SUTO Chikako   須藤　史子    所属   医学部 医学科（附属足立医療センター）    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読なし
表題	Haze development after photorefractive keratectomy: mechanical vs ethanol epithelial removal in rabbits.
掲載誌名	正式名：Journal of refractive surgery (Thorofare, N.J. : 1995) 略　 称：J Refract Surg ISSNコード：1081597X/1081597X
巻・号・頁	24(9),pp.923-927
著者・共著者	de Medeiros Fabricio Witzel†, Mohan Rajiv R, Suto Chikako, Sinhá Sunilima, Bonilha Vera L, Chaurasia Shyam S, Wilson Steven E
発行年月	2008/11
概要	PURPOSE:To compare mechanical and ethanol epithelial removal with respect to myofibroblast development and haze formation after photorefractive keratectomy (PRK).METHODS:Seventeen rabbits underwent mechanical or ethanol debridement, and the opposite eye of each rabbit served as an unwounded control. In both groups, the epithelium was removed with a spatula and discarded. A -9.00-diopter PRK was performed in each eye. The level of haze in each cornea at 4 weeks was graded at the slit-lamp microscope according to the Fantes scale. Myofibroblast generation was detected with immunocytochemistry for alpha-smooth muscle actin (alpha-SMA) and cells were quantitatively analyzed.RESULTS:No difference was noted between the two groups in alpha-SMA + myofibroblasts 4 weeks after surgery (43.6+/-2.0/400x field and 45.7+/-4.8/400x field in ethanol and mechanical groups, respectively) (P=.10). A slight difference was noted but did not reach statistical significance with regard to stromal haze between ethanol and mechanical groups (2.0+/-0.5 and 2.3+/-0.4, respectively, P=.063). The ethanol and mechanical groups were statistically different when compared to controls regarding stromal haze and alpha-SMA+ cells (P<.0001for all comparisons).CONCLUSIONS:No difference was noted in clinical haze or myofibroblast generation between corneas that had PRK with mechanical or ethanol epithelial debridement.
DOI	10.3928/1081597X-20081101-11
PMID	19044233