ヤマト　マサユキ   YAMATO Masayuki   大和　雅之    所属   研究施設 研究施設    職種   教授
論文種別	その他
言語種別	英語
査読の有無	査読なし
表題	Fabrication of transplantable corneal epithelial and oral mucosal epithelial cell sheets using a novel temperature-responsive closed culture device.
掲載誌名	正式名：Journal of tissue engineering and regenerative medicine 略　 称：J Tissue Eng Regen Med ISSNコード：19327005/19326254
掲載区分	国外
巻・号・頁	9(5),pp.637-640
著者・共著者	NAKAJIMA Ryota†, KOBAYASHI Toyoshige, KIKUCHI Tetsutaro, KITANO Yuriko, WATANABE Hiroya, MIZUTANI Manabu, NOZAKI Takayuki, SENDA Naoko, SAITO Kazuo, TAKAGI Ryo, YAMATO Masayuki, OKANO Teruo, TAKEDA Shizu*
発行年月	2015/05
概要	Temperature-responsive culture surfaces make it possible to harvest transplantable carrier-free cell sheets. Here, we applied temperature-responsive polymer for polycarbonate surfaces with previously developed closed culture devices for an automated culture system in order to fabricate transplantable stratified epithelial cell sheets. Histological and immunohistochemical analyses and colony-forming assays revealed that corneal epithelial and oral mucosal epithelial cell sheets could be harvested with the temperature-responsive closed culture devices. The results were similar to those obtained using temperature-responsive culture inserts. These results indicate that the novel temperature-responsive closed culture device is useful for fabricating transplantable stratified epithelial cell sheets.
DOI	10.1002/term.1728
PMID	23475606