YAMATO Masayuki
Department Research Institutes and Facilities, Research Institutes and Facilities Position Professor |
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Article types | Original article |
Language | English |
Peer review | Peer reviewed |
Title | Water Stable Nanocoatings of Poly(N-isopropylacrylamide)-based Block Copolymers on Culture Insert Membrane for Temperature-Controlled Cell Adhesion |
Journal | Formal name:Journal of materials chemistry. B. Abbreviation:J Mater Chem B ISSN code:2050750X/20507518 |
Domestic / Foregin | Foregin |
Publisher | Royal Society of Chemistry |
Volume, Issue, Page | 8(34),pp.7812-7821 |
Author and coauthor | NAKAYAMA Masamichi†*, TOYOSHIMA Yuki, CHINEN Hiroshi, KIKUCHI Akihiko, YAMATO Masayuki, OKANO Teruo |
Publication date | 2020/07/17 |
Summary | This study demonstrated the spin-coating of functional diblock copolymers to develop smart culture inserts for thermoresponsive cell adhesion/detachment control. One part of the block components, the poly(n-butyl methacrylate) block, strongly supported the water stable surface-immobilization of the thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm) block, regardless of temperature. The chain length of the PNIPAAm blocks was varied to regulate thermal surface functions. Immobilized PNIPAAm concentrations became larger with increasing chain length (1.0–1.6 μg cm−2) and the thicknesses of individual layers were relatively comparable at 10-odd nanometers. A nanothin coating scarcely inhibited the permeability of the original porous membrane. When human fibroblasts were cultured on each surface at 37 °C, the efficiencies of cell adhesion and proliferation decreased with longer PNIPAAm chains. Meanwhile, by reducing the temperature to 20 °C, longer PNIPAAm chains promoted cell detachment owing to the significant thermoresponsive alteration of cell-surface affinity. Consequently, we successfully produced a favorable cell sheet by choosing an appropriate PNIPAAm length for block copolymers. |
DOI | 10.1039/D0TB01113D |