ヤマト マサユキ   YAMATO Masayuki
  大和 雅之
   所属   研究施設 研究施設
   職種   教授
論文種別 その他
言語種別 英語
査読の有無 査読あり
表題 Lessons learned from conventional animals: Encouragement to use specific-pathogen-free animals.
掲載誌名 正式名:Regenerative Therapy
ISSNコード:2352-3204
掲載区分国外
出版社 Science Direct
巻・号・頁 14,296-298頁
著者・共著者 MORINO Tsunetaro†, KIKUCHI Shun, INAGAKI Taro, KOMORI Manabu, YAMAMOTO Kazuhisa, KOJIMA Hiromi, YAMATO Masayuki*
発行年月 2020/06
概要 Experimental rabbits provide evidence for translational research regarding the pathogenies or treatment of human diseases. We developed a novel method for regenerating the middle ear mucosa using autologous cultured nasal mucosal epithelial cell sheets, and evaluated the wound healing process in the middle ear mucosa of experimental rabbits. Nonetheless, vigilant microbiological monitoring of experimental animals is essential to effectively prevent a decline in their health conditions, which may affect the research results. We experimented with contamination of Pasteurella multocida in non-specific-pathogen-free (SPF) rabbits (without microbiological monitoring). Most non-SPF rabbits had otitis media, whereas SPF rabbits did not, which affected their results during the mucosal regeneration study. The contamination was resolved by changing the experimental design from using non-SPF rabbits to that using SPF rabbits. It is crucial to use the SPF animals for any surgical intervention studies.
DOI 10.1016/j.reth.2020.03.003
PMID 32455159