YAMATO Masayuki
Department Research Institutes and Facilities, Research Institutes and Facilities Position Professor |
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Article types | Original article |
Language | English |
Peer review | Peer reviewed |
Title | Type I collagen-induced YAP nuclear expression promotes primary cilia growth and contributes to cell migration in confluent mouse embryo fibroblast 3T3-L1 cells. |
Journal | Formal name:Molecular and cellular biochemistry Abbreviation:Mol Cell Biochem ISSN code:(1573-4919)0300-8177(Linking) |
Domestic / Foregin | Foregin |
Publisher | Springer |
Volume, Issue, Page | 450(1-2),pp.87-96 |
Author and coauthor | XU Qian†, LIU Xiaoling, LIU Weiwei, HAYASHI Toshihiko, YAMATO Masayuki, FUJISAKI Hitomi, HATTORI Shunji, TASHIRO Shin-Ichi, ONODERA Satoshi, IKEJIMA Takashi* |
Publication date | 2019/01 |
Summary | The extracellular matrix (ECM) is a major biomechanical environment for all cells in vivo, and tightly controls wound healing and cancer progression. Type I collagen (Col I) is the most abundant component in ECM and plays an essential role for cell motility control and migration beyond structural support. Our previous results showed that Col I increased the length of primary cilia and the expression of primary cilia-associated proteins in 3T3-L1 cells. The Hippo/YAP pathway serves as a major integrator of cell surface-mediated signals and regulates key processes for the development and maintenance of tissue functions. In this study, we investigated the role of Hippo/YAP signaling in primary cilia growth of cells cultured on Col I-coated plate, as well as the potential link between primary cilia and migration. At 2-day post-confluence, YAP localization in the nucleus was dramatically increased when the cells were cultured on Col I-coated plate, accompanied by cilia growth. YAP inhibitor verteporfin repressed the growth of primary cilia as well as the expressions of ciliogenesis-associated proteins in confluent 3T3-L1 cells cultured on Col I-coated plate. Moreover, knockdown of either YAP or IFT88, one of the ciliogenesis-associated proteins, reversed the migration of confluent 3T3-L1 cells promoted by Col I-coating. In conclusion, activation of YAP pathway by Col I-coating of culture plate for confluent 3T3-L1 cells is positively associated with the primary cilia growth, which eventually results in promoted migration. |
DOI | 10.1007/s11010-018-3375-z |
PMID | 29846859 |