ヤマト　マサユキ   YAMATO Masayuki   大和　雅之    所属   研究施設 研究施設    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Enrichment of corneal epithelial stem/progenitor cells using cell surface markers, integrin alpha6 and CD71.
掲載誌名	正式名：Biochemical and biophysical research communications 略　 称：Biochem Biophys Res Commun ISSNコード：(1090-2104)0006-291X(Linking)
掲載区分	国外
巻・号・頁	367(2),pp.256-63
著者・共著者	Hayashi Ryuhei†, Yamato Masayuki, Saito Teiko, Oshima Tetsuro, Okano Teruo, Tano Yasuo, Nishida Kohji*
発行年月	2008/03
概要	Corneal epithelial stem cells are believed to reside in the basal layer of the limbal epithelium, but no definitive cell surface markers have been identified. For keratinocytes, stem/progenitor cells are known to be enriched by cell surface markers, integrin alpha(6) and CD71, as a minor subpopulation which shows high integrin alpha(6) and low CD71 expressions (alpha(6)(bri)/CD71(dim)). In the present study, we investigated the possibility that corneal epithelial stem cells can be enriched by integrin alpha(6) and CD71. The alpha(6)(bri)/CD71(dim) cells were separated by fluorescence-activated cell sorting, as a minor subpopulation of the limbal epithelial cells. They were enriched for relatively small cells, showing a higher clonogenic capacity and expression of stem cell markers, but a lower expression of differentiation markers, compared to other cell populations. The cells were localized immunohistochemically in the basal region of the limbal epithelium. These results indicate that the alpha(6)(bri)/CD71(dim) subpopulation enriched corneal epithelial stem cells.
DOI	10.1016/j.bbrc.2007.12.077
PMID	18155160