ヤマト マサユキ   YAMATO Masayuki
  大和 雅之
   所属   研究施設 研究施設
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 The effect of extensible PEG tethers on shielding between grafted thermo-responsive polymer chains and integrin-RGD binding.
掲載誌名 正式名:Biomaterials
略  称:Biomaterials
ISSNコード:(0142-9612)0142-9612(Linking)
掲載区分国外
巻・号・頁 29(27),3650-5頁
著者・共著者 Ebara Mitsuhiro†, Yamato Masayuki, Aoyagi Takao, Kikuchi Akihiko, Sakai Kiyotaka, Okano Teruo*
発行年月 2008/09
概要 The affinity control of integrin-RGD (Arg-Gly-Asp) binding by a thermal "on-off" switch has been achieved using newly designed surfaces presenting grafted temperature-responsive poly(N-isopropylacrylamide-co-2-carboxyisopropylacrylamide) copolymers functionalized with synthetic peptides. The prepared surface was designed to expose the tethered peptides available for cell binding at active "on" state above the lower critical solution temperature (LCST). The fully extended chains, on the other hand, masked the peptides completely and the cells started to detach from the surfaces at inactive "off" sate below the LCST. This paper elucidates the shielding effect of the grafted polymer chains on the dissociation of integrin-RGD binding below the LCST. To assess the ability of the polymer-shielding, extensible poly(ethylene glycol) (PEG) tethers were introduced between peptides and the grafted polymers. PEG chains allow peptides to be tethered to surfaces via functional PEG end-groups, leading to active "on" state even below the LCST. The time required to release cells from the surface was found to be longer when peptides were coupled to an extensible tether ends, suggesting that the surfaces can engender cell attachment through adhesive moieties covalently bound to the free ends of PEG chains. These results indicate that architectural changes on the nanometer length scale are crucial for controlling integrin-RGD binding and one of the main factors causing cell detachment is the shielding effect of the grafted polymer chains.
DOI 10.1016/j.biomaterials.2008.05.030
PMID 18582933