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YAMATO Masayuki
Department Research Institutes and Facilities, Research Institutes and Facilities Position Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | CD61 enriches long-term repopulating hematopoietic stem cells. |
| Journal | Formal name：Biochemical and biophysical research communications Abbreviation：Biochem Biophys Res Commun ISSN code：(1090-2104)0006-291X(Linking) |
| Domestic / Foregin | Foregin |
| Publisher | Elsevier |
| Volume, Issue, Page | 365(1),pp.176-182 |
| Author and coauthor | Umemoto Terumasa†, Yamato Masayuki, Shiratsuchi Yoshiko, Terasawa Masao, Yang Joseph, Nishida Kohji, Kobayashi Yoshiro, Okano Teruo* |
| Authorship | 2nd author |
| Publication date | 2008/01 |
| Summary | Among the subsets that define hematopoietic stem cells (HSCs), CD34- c-kit+ Sca-1+ lineage marker- (CD34-KSL) cells are regarded as one of the populations that have the highest enrichment of HSCs in adult mouse bone marrow. Here, we demonstrate that long-term repopulating hematopoietic stem cells (LTR-HSCs) have high expression of CD61 (integrin beta3) within the CD34-KSL population. Approximately 60% of CD34-KSL cells showed high expression of CD61. CD61HighCD34-KSL populations also exhibited significantly greater properties of HSC, such as expression of HSC markers, the side population (SP) phenotype, and ability for long-term repopulation. In both SP cells and non-SP (NSP) cells, CD61HighCD34-KSL cells also contained significantly more LTR-HSCs than CD61Low/-CD34-KSL cells. Our results indicate that CD61 is exploitable for HSC enrichment as a supportive positive cell surface marker. |
| DOI | 10.1016/j.bbrc.2007.10.168 |
| PMID | 17983596 |