ヤマト マサユキ   YAMATO Masayuki
  大和 雅之
   所属   研究施設 研究施設
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 CD61 enriches long-term repopulating hematopoietic stem cells.
掲載誌名 正式名:Biochemical and biophysical research communications
略  称:Biochem Biophys Res Commun
ISSNコード:(1090-2104)0006-291X(Linking)
掲載区分国外
出版社 Elsevier
巻・号・頁 365(1),176-182頁
著者・共著者 Umemoto Terumasa†, Yamato Masayuki, Shiratsuchi Yoshiko, Terasawa Masao, Yang Joseph, Nishida Kohji, Kobayashi Yoshiro, Okano Teruo*
発行年月 2008/01
概要 Among the subsets that define hematopoietic stem cells (HSCs), CD34- c-kit+ Sca-1+ lineage marker- (CD34-KSL) cells are regarded as one of the populations that have the highest enrichment of HSCs in adult mouse bone marrow. Here, we demonstrate that long-term repopulating hematopoietic stem cells (LTR-HSCs) have high expression of CD61 (integrin beta3) within the CD34-KSL population. Approximately 60% of CD34-KSL cells showed high expression of CD61. CD61HighCD34-KSL populations also exhibited significantly greater properties of HSC, such as expression of HSC markers, the side population (SP) phenotype, and ability for long-term repopulation. In both SP cells and non-SP (NSP) cells, CD61HighCD34-KSL cells also contained significantly more LTR-HSCs than CD61Low/-CD34-KSL cells. Our results indicate that CD61 is exploitable for HSC enrichment as a supportive positive cell surface marker.
DOI 10.1016/j.bbrc.2007.10.168
PMID 17983596