YAMATO Masayuki
   Department   Research Institutes and Facilities, Research Institutes and Facilities
   Position   Professor
Article types Original article
Language English
Peer review Peer reviewed
Title Differential levels of reactive oxygen species in murine preadipocyte 3T3-L1 cells cultured on type I collagen molecule-coated and gel-covered dishes exert opposite effects on NF-κB-mediated proliferation and migration.
Journal Formal name：Free radical research
Abbreviation：Free Radic Res
ISSN code：(1029-2470)1029-2470(Linking)
Domestic / ForeginForegin
Publisher Taylor & Francis
Volume, Issue, Page 52(9),pp.913-928
Author and coauthor LIU Xiaoling†, LONG Xinyu, LIU Weiwei, YAO Guodong, ZHAO Yeli, HAYASHI Toshihiko, HATTORI Shunji, FUJISAKI Hitomi, OGURA Takaaki, TASHIRO Shin-Ichi, ONODERA Satoshi, YAMATO Masayuki, IKEJIMA Takashi*
Publication date 2018/09
Summary Reactive oxygen species (ROS) participate in various cell responses in association with cell proliferation, migration, differentiation and death. Extracellular matrix (ECM) serves as cellular microenvironments for many kinds of cells, affecting cell activities. However, whether or not ECM influences cellular ROS levels has not been well studied. In this study, cells are cultured on collagen I molecule-coated (mol. coated) dishes and collagen I fibrous gel-covered (gel) dishes to explore their influence on cell behaviors. We found that the levels of ROS in murine 3T3-L1 preadipocytes increased both in cells on mol. coated and those on gel. Much higher ROS levels were found in the cells cultured on gel. Cell proliferation and migration were stimulated to opposite directions between the cells on mol. coated and the cells on gel. ROS in a moderate level were positive regulators in the proliferation and migration of cells on mol. coated; however, ROS in a high level served as negative regulators in the cells on gel. These opposite effects on cell proliferation and migration affected by different ROS levels are in parallel with opposite levels of NF-κB p65 activation.
DOI 10.1080/10715762.2018.1478088
PMID 29768932