ヤマト　マサユキ   Yamato Masayuki   大和　雅之    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Differential levels of reactive oxygen species in murine preadipocyte 3T3-L1 cells cultured on type I collagen molecule-coated and gel-covered dishes exert opposite effects on NF-κB-mediated proliferation and migration.
掲載誌名	正式名：Free radical research 略　 称：Free Radic Res ISSNコード：(1029-2470)1029-2470(Linking)
掲載区分	国外
出版社	Taylor & Francis
巻・号・頁	52(9),pp.913-928
著者・共著者	LIU Xiaoling†, LONG Xinyu, LIU Weiwei, YAO Guodong, ZHAO Yeli, HAYASHI Toshihiko, HATTORI Shunji, FUJISAKI Hitomi, OGURA Takaaki, TASHIRO Shin-Ichi, ONODERA Satoshi, YAMATO Masayuki, IKEJIMA Takashi*
発行年月	2018/09
概要	Reactive oxygen species (ROS) participate in various cell responses in association with cell proliferation, migration, differentiation and death. Extracellular matrix (ECM) serves as cellular microenvironments for many kinds of cells, affecting cell activities. However, whether or not ECM influences cellular ROS levels has not been well studied. In this study, cells are cultured on collagen I molecule-coated (mol. coated) dishes and collagen I fibrous gel-covered (gel) dishes to explore their influence on cell behaviors. We found that the levels of ROS in murine 3T3-L1 preadipocytes increased both in cells on mol. coated and those on gel. Much higher ROS levels were found in the cells cultured on gel. Cell proliferation and migration were stimulated to opposite directions between the cells on mol. coated and the cells on gel. ROS in a moderate level were positive regulators in the proliferation and migration of cells on mol. coated; however, ROS in a high level served as negative regulators in the cells on gel. These opposite effects on cell proliferation and migration affected by different ROS levels are in parallel with opposite levels of NF-κB p65 activation.
DOI	10.1080/10715762.2018.1478088
PMID	29768932