ヤマト マサユキ   YAMATO Masayuki
  大和 雅之
   所属   研究施設 研究施設
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Gelatin promotes cell aggregation and pro-inflammatory cytokine production in PMA-stimulated U937 cells by augmenting endocytosis-autophagy pathway.
掲載誌名 正式名:The international journal of biochemistry & cell biology
略  称:Int J Biochem Cell Biol
ISSNコード:(1878-5875)1357-2725(Linking)
掲載区分国外
出版社 Elsevier
巻・号・頁 95,132-142頁
著者・共著者 ZHAO Ye-Li†, LU Zhuo-Yu, ZHANG Xuan, LIU Wei-Wei, YAO Guo-Dong, LIU Xiao-Ling, LIU Wei, WU Qing-Jie, HAYASHI Toshihiko, YAMATO Masayuki, FUJISAKI Hitomi, HATTORI Shunji, ATSUZAWA Yuji, TASHIRO Shin-Ichi, ONODERA Satoshi, IKEJIMA Takashi*
発行年月 2018/01
概要 Gelatin, denatured collagen, temporarily exists in tissues and may well be pathophysiologically involved in tissue remodeling, inflammation or tissue damage. The present study is aimed to investigate possible biological roles of gelatin by examining its effects on monocyte-like histiocytic lymphoma cell line U937. Once stimulated by phorbol 12-myristate 13-acetate (PMA), U937 cells differentiate into macrophage-like cells, changing from non-adherent to adherent cells with extended pseudopodia. Here we pre-treated the cell dishes with gelatin solution for cell culture. Interestingly, we found that PMA-stimulated U937 cells formed multicellular aggregates on gelatin-coated dishes, accompanying NF-κB-mediated production of pro-inflammatory cytokines, whereas cell aggregation was not detected on non-coated dishes. Moreover, differentiated U937 cells on gelatin-coated dishes showed increased autophagy level and endocytosis. Surprisingly, formation of multicellular aggregates and pro-inflammatory cytokine production were both attenuated by either down-regulation of autophagy with inhibitors, such as 3-methyladenine (3MA) or chloroquine (CQ), or repression of endocytosis with siRNA targeting Endo180. Moreover, autophagy was inhibited by si-Endo180, and endocytosis was suppressed by 3MA, suggesting a positive feedback loop between autophagy and endocytosis. The results revealed that gelatin-coating induced differentiated U937 cells to form cell aggregates and promote NF-κB-mediated pro-inflammatory cytokine production at least partially through an endocytosis-autophagy pathway.
DOI 10.1016/j.biocel.2018.01.002
PMID 29307663