ヤマト マサユキ   YAMATO Masayuki
  大和 雅之
   所属   研究施設 研究施設
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets.
掲載誌名 正式名:Macromolecular bioscience
略  称:Macromol Biosci
ISSNコード:(1616-5195)1616-5187(Linking)
掲載区分国外
出版社 Wiley
巻・号・頁 17(8),1700073頁
著者・共著者 Nagase Kenichi†, Nagumo Yuhei, Kim Miri, Kim Hee-Jung, Kyung Hei-Won, Chung Hye-Jin, Sekine Hidekazu, Shimizu Tatsuya, Kanazawa Hideko, Okano Teruo, Lee Seung-Jin, Yamato Masayuki*
発行年月 2017/05
概要 Cell sheet transplantation is a key tissue engineering technology. A vascular endothelial growth factor (VEGF)-releasing fiber mat is developed for the transplantation of multilayered cardiomyocyte sheets. Poly(vinyl alcohol) fiber mats bearing poly(lactic-co-glycolic acid) nanoparticles that incorporate VEGF are fabricated using electrospinning and electrospray methods. Six-layered cardiomyocyte sheets are transplanted with a VEGF-releasing mat into athymic rats. After two weeks, these sheets produce thicker cardiomyocyte layers compared with controls lacking a VEGF-releasing mat, and incorporate larger-diameter blood vessels containing erythrocytes. Thus, local VEGF release near the transplanted cardiomyocytes induces vascularization, which supplies sufficient oxygen and nutrients to prevent necrosis. In contrast, cardiomyocyte sheets without a VEGF-releasing mat do not survive in vivo, probably undergo necrosis, and are reduced in thickness. Hence, these VEGF-releasing mats enable the transplantation of multilayered cardiomyocyte sheets in a single procedure, and should expand the potential of cell sheet transplantation for therapeutic applications.
DOI 10.1002/mabi.201700073
PMID 28547766