ヤマト マサユキ   YAMATO Masayuki
  大和 雅之
   所属   研究施設 研究施設
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Significantly different proliferative potential of oral mucosal epithelial cells between six animal species.
掲載誌名 正式名:Journal of biomedical materials research. Part A
略  称:J Biomed Mater Res A
ISSNコード:(1552-4965)1549-3296(Linking)
掲載区分国外
巻・号・頁 102(6),1829-37頁
著者・共著者 Kondo Makoto†, Yamato Masayuki, Takagi Ryo, Murakami Daisuke, Namiki Hideo, Okano Teruo*
発行年月 2014/06
概要 There has been an upsurge in regenerative medicine in recent years. In particular, because oral mucosal epithelial cells can be obtained noninvasively, cultured epithelial cell sheets have been used in a number of ectopic transplantations. Additionally, the verification of the properties of experimental animals' cultured cells has accelerated the application of regenerative medicine. In the present study, the properties of oral mucosal epithelial cells were compared between six animal species. The human and pig epithelia were relatively thicker than the epithelia of the other species. The colony-forming efficiency of the rat was the highest, followed by those of the dog, human, rabbit, and pig, whereas the colonies of the mouse cells were all paraclone and uncountable in the colony-forming assay. We also found that the rabbit and pig cells proliferated poorly and were unable to form cell sheets without feeder layers. In contrast, even in the absence of feeder layers and cholera toxin, cultured dog and mouse cells formed contiguous sheets, when the cell seeding density was high. These results indicate that interspecies variation is considerable in oral mucosal epithelial cells and that specific experimental animal or human cells must be chosen according to the intended use.
DOI 10.1002/jbm.a.34849
PMID 23818153