ヤマト　マサユキ   YAMATO Masayuki   大和　雅之    所属   研究施設 研究施設    職種   教授
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Cartilage repair in transplanted scaffold-free chondrocyte sheets using a minipig model.
Journal	Formal name：Biomaterials Abbreviation：Biomaterials ISSN code：1878-5905(Electronic)0142-9612(Linking)
Volume, Issue, Page	33(15),pp.3846-51
Author and coauthor	Ebihara Goro†, Sato Masato*, Yamato Masayuki, Mitani Genya, Kutsuna Toshiharu, Nagai Toshihiro, Ito Satoshi, Ukai Taku, Kobayashi Miyuki, Kokubo Mami, Okano Teruo, Mochida Joji
Publication date	2012/05
Summary	Lacking a blood supply and having a low cellular density, articular cartilage has a minimal ability for self-repair. Therefore, wide-ranging cartilage damage rarely resolves spontaneously. Cartilage damage is typically treated by chondrocyte transplantation, mosaicplasty or microfracture. Recent advances in tissue engineering have prompted research on techniques to repair articular cartilage damage using a variety of transplanted cells. We studied the repair and regeneration of cartilage damage using layered chondrocyte sheets prepared on a temperature-responsive culture dish. We previously reported achieving robust tissue repair when covering only the surface layer with layered chondrocyte sheets when researching partial-thickness defects in the articular cartilage of domestic rabbits. The present study was an experiment on the repair and regeneration of articular cartilage in a minipig model of full-thickness defects. Good safranin-O staining and integration with surrounding tissues was achieved in animals transplanted with layered chondrocyte sheets. However, tissue having poor safranin-O staining-not noted in the domestic rabbit experiments-was identified in some of the animals, and the subchondral bone was poorly repaired in these. Thus, although layered chondrocyte sheets facilitate articular cartilage repair, further investigations into appropriate animal models and culture and transplant conditions are required.
DOI	10.1016/j.biomaterials.2012.01.056
Document No.	22369960