ヤマト マサユキ   YAMATO Masayuki
  大和 雅之
   所属   研究施設 研究施設
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Repair of articular cartilage defect with layered chondrocyte sheets and cultured synovial cells.
掲載誌名 正式名:Biomaterials
略  称:Biomaterials
ISSNコード:1878-5905(Electronic)0142-9612(Linking)
巻・号・頁 33(21),5278-86頁
著者・共著者 Ito Satoshi†, Sato Masato*, Yamato Masayuki, Mitani Genya, Kutsuna Toshiharu, Nagai Toshihiro, Ukai Taku, Kobayashi Miyuki, Kokubo Mami, Okano Teruo, Mochida Joji
発行年月 2012/07
概要 In this study, we investigate the effects of treatment with layered chondrocyte sheets and synovial cell transplantation. An osteochondral defect was created of 48 Japanese white rabbits. In order to determine the effects of treatment, the following 6 groups were produced: (A) synovial cells (1.8 × 10(6) cells), (B)layered chondrocyte sheets (1.7 × 10(6) cells), (C) synovial cells (3.0 × 10(5) cells) + layered chondrocyte sheets, (D)synovial cells (6.0 × 10(5) cells) + layered chondrocyte sheets, (E)synovial cells (1.2 × 10(6) cells) + layered chondrocyte sheets, (F) osteochondral defect. Layered chondrocyte sheets and synovial cells were transplanted, sacrificed four and 12 weeks postoperatively. An incapacitance tester (Linton) was used to find trends in the weight distribution ratio of the damaged limbs after surgery. Sections were stained with Safranin-O. Repair sites were evaluated using ICRS grading system. In groups (A) to (E), the damaged limb weight distribution ratio had improved. The repair tissue stained positively with Safranin-O. Four and 12 weeks after surgery, groups (A) to (E) exhibited significantly higher scores than group (F), and groups (D) and (E) exhibited significantly higher scores than groups (A) and (B). This suggests the efficacy of combining layered chondrocyte sheets with synovial cells.
DOI 10.1016/j.biomaterials.2012.03.073
文献番号 22551484