Michio Otsuki    Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine    Position   Professor and Division head
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Ketone body 3-hydroxybutyrate enhances adipocyte function.
Journal	Formal name：Scientific reports Abbreviation：Sci Rep ISSN code：20452322/20452322
Domestic / Foregin	Foregin
Volume, Issue, Page	12(1),pp.10080
Author and coauthor	Nishitani Shigeki, Fukuhara Atsunori, Tomita Issei, Kume Shinji, Shin Jihoon, Okuno Yosuke, Otsuki Michio, Maegawa Hiroshi, Shimomura Iichiro
Publication date	2022/06
Summary	Ketone bodies, including 3HBA, are endogenous products of fatty acid oxidation, and Hmgcs2 is the first rate-limiting enzyme of ketogenesis. From database analysis and in vivo and in vitro experiments, we found that adipose tissue and adipocytes express Hmgcs2, and that adipocytes produce and secrete 3HBA. Treatment with 3HBA enhanced the gene expression levels of the antioxidative stress factors, PPARγ, and lipogenic factors in adipose tissue in vivo and in adipocytes in vitro, accompanied by reduced ROS levels. Knockdown of endogenous Hmgcs2 in adipocytes markedly decreased 3HBA levels in adipocytes and decreased the gene expression levels of the antioxidative stress factors, PPARγ, and lipogenic factors with increased ROS levels. Conversely, overexpression of Hmgcs2 in adipocytes increased 3HBA secretion from adipocytes and enhanced the gene expression levels of the antioxidative stress factors, PPARγ, and lipogenic factors. These results demonstrate that 3HBA plays significant roles in enhancing the physiological function of adipocytes.
DOI	10.1038/s41598-022-14268-w
PMID	35710581