Michio Otsuki    Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine    Position   Professor and Division head
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Specific regulation of lipocalin-type prostaglandin D synthase in mouse heart by estrogen receptor beta
Journal	Formal name：Molecular endocrinology Abbreviation：Mol Endocrinol ISSN code：08888809 (Print)08888809 (Linking)
Domestic / Foregin	Foregin
Volume, Issue, Page	17(9),pp.1844-1855
Author and coauthor	Otsuki, M. Gao, H. Dahlman-Wright, K. Ohlsson, C. Eguchi, N. Urade, Y. Gustafsson, J. A.
Authorship	Lead author
Publication date	2003
Summary	Estrogens have important physiological roles in the cardiovascular system. We use DNA microarray technology to study the molecular mechanism of estrogen action in the heart and to identify novel estrogen-regulated genes. In this investigation we identify genes that are regulated by chronic estrogen treatment of mouse heart. We present our detailed characterization of one of these genes, lipocalin-type prostaglandin D synthase (L-PGDS). Northern and Western blot analysis revealed that L-PGDS was induced both by acute and chronic estrogen treatment. Northern blot analysis, using estrogen receptor (ER)-disrupted mice, suggests that L-PGDS is specifically induced by ERbeta in vivo. In further support of ERbeta-selective regulation, we identify a functional estrogen-responsive element in the L-PGDS promoter, the activity of which is up-regulated by ERbeta, but not by ERalpha. We demonstrate that a one-nucleotide change (A to C) in the L-PGDS estrogen-responsive element affects receptor selectivity.
DOI	10.1210/me.2003-0016
Document No.	12829806