オオツキ　ミチオ   Michio Otsuki   大月　道夫    所属   医学部 医学科（東京女子医科大学病院）    職種   教授・基幹分野長
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Specific regulation of lipocalin-type prostaglandin D synthase in mouse heart by estrogen receptor beta
掲載誌名	正式名：Molecular endocrinology 略　 称：Mol Endocrinol ISSNコード：08888809 (Print)08888809 (Linking)
掲載区分	国外
巻・号・頁	17(9),pp.1844-1855
著者・共著者	Otsuki, M. Gao, H. Dahlman-Wright, K. Ohlsson, C. Eguchi, N. Urade, Y. Gustafsson, J. A.
担当区分	筆頭著者
発行年月	2003
概要	Estrogens have important physiological roles in the cardiovascular system. We use DNA microarray technology to study the molecular mechanism of estrogen action in the heart and to identify novel estrogen-regulated genes. In this investigation we identify genes that are regulated by chronic estrogen treatment of mouse heart. We present our detailed characterization of one of these genes, lipocalin-type prostaglandin D synthase (L-PGDS). Northern and Western blot analysis revealed that L-PGDS was induced both by acute and chronic estrogen treatment. Northern blot analysis, using estrogen receptor (ER)-disrupted mice, suggests that L-PGDS is specifically induced by ERbeta in vivo. In further support of ERbeta-selective regulation, we identify a functional estrogen-responsive element in the L-PGDS promoter, the activity of which is up-regulated by ERbeta, but not by ERalpha. We demonstrate that a one-nucleotide change (A to C) in the L-PGDS estrogen-responsive element affects receptor selectivity.
DOI	10.1210/me.2003-0016
文献番号	12829806