Michio Otsuki    Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine    Position   Professor and Division head
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Regeneration of tubular complex is promoted by a free space
Journal	Formal name：Pancreas Abbreviation：Pancreas ISSN code：15364828 (Electronic)08853177 (Linking)
Domestic / Foregin	Foregin
Volume, Issue, Page	30(2),pp.174-179
Author and coauthor	Kitamura, T. Asanuma, N. Inaba, M. Otsuki, M. Kasayama, S. Kouhara, H. Kawase, I.
Publication date	2005
Summary	OBJECTIVES: Regeneration of the pancreas is initiated by the tubular complexes that consist of a cluster of epithelia surrounded by the mesenchymal cells. They have the potential to become pancreatic lobes, but their growth stops before the complete regeneration of the organ. To elucidate the possibility that we could promote the regeneration of the pancreas, the potential for growth or differentiation of tubular complex was analyzed. METHODS: The intact lobes were growing around the silk knot after ligation of the pancreas in adult mice. To develop this reaction to a quantitative assay, tubular complexes were induced on the silk strings in the pancreas and were growing into a free space under the silicon cover. The proliferation and differentiation of new lobes with or without the space were analyzed. RESULTS: The number of tubular complexes, which express PDX-1, was increased 5.4 times by the space effect. The proliferating cell nuclear antigen labeling index of acinar cells was 1.7 times stimulated, but that of tubular complex was not changed. The amputated pancreas recovered 49.5% of the resected part under the silicon cover; however, it remained the same weight without the cover. CONCLUSION: The proliferation and differentiation of tubular complex are promoted by a free space.
DOI	10.1097/01.mpa.0000150110.45017.fc.
Document No.	15714140