Michio Otsuki
   Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine
   Position   Professor and Division head
Article types Original article
Language English
Peer review Peer reviewed
Title The thiazolidinedione drug troglitazone up-regulates nitric oxide synthase expression in vascular endothelial cells
Journal Formal name：Journal of diabetes and its complications
Abbreviation：J Diabetes Complications
ISSN code：10568727 (Print)10568727 (Linking)
Domestic / ForeginForegin
Volume, Issue, Page 20(5),pp.336-342
Author and coauthor Goya, K. Sumitani, S. Otsuki, M. Xu, X. Yamamoto, H. Kurebayashi, S. Saito, H. Kouhara, H. Kasayama, S.
Publication date 2006
Summary Endothelial dysfunction is a phenomenon often observed in diabetic patients, which is a cause for vascular complications of diabetes mellitus. Endothelium-derived nitric oxide (NO) is responsible for vasodilatation, and NO-dependent vasodilatation is diminished in diabetic patients. In the present study, we evaluated the effects of thiazolidinediones (TZDs), antidiabetic drugs known to improve insulin resistance and to have vasodilating properties, on endothelial NO synthase (eNOS) expression in cultured vascular endothelial cells. Human umbilical vein endothelial cells were treated with the TZDs troglitazone and pioglitazone, or the peroxisome proliferator-activated receptor (PPAR) gamma activator 15-deoxy-Delta(12,14)-prostaglandin J(2) (15-dPGJ2). The expression of eNOS protein and its mRNA was determined by Western and Northern blot analyses, respectively. The effect of alpha-tocopherol that possesses structural similarity to troglitazone was also examined. Troglitazone up-regulated eNOS protein and its mRNA levels, whereas pioglitazone and 15-dPGJ2 failed to increase their levels. By contrast, alpha-tocopherol also increased in eNOS protein and mRNA. These results suggest that troglitazone up-regulates eNOS expression probably through its 6-hydroxychromanes structure but not activating PPARgamma.
DOI 10.1016/j.jdiacomp.2005.08.003
Document No. 16949522