MORIMOTO Satoshi
   Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine
   Position   Associate Professor
Article types Original article
Language English
Peer review Peer reviewed
Title Thyroid hormone activation in vascular smooth muscle cells is negatively regulated by glucocorticoid.
Journal Formal name：Thyroid : official journal of the American Thyroid Association
Abbreviation：Thyroid
ISSN code：15579077/10507256
Domestic / ForeginForegin
Volume, Issue, Page 19(7),pp.755-63
Author and coauthor Toyoda Nagaoki, Yasuzawa-Amano Saori, Nomura Emiko, Yamauchi Azusa, Nishimura Kumiko, Ukita Chizuko, Morimoto Satoshi, Kosaki Atsushi, Iwasaka Toshiji, Harney John W, Larsen P Reed, Nishikawa Mitsushige
Publication date 2009/07
Summary BACKGROUND:Type 2 iodothyronine deiodinase (D2) catalyzes the production of triiodothyronine from thyroxine. D2 is present in rat aorta media, and there is a circadian variation in the D2 expression. In rat aorta media, the D2 activity exhibited the maximal value at 1200 hour and low value between 1800 and 2400 hour. To understand the mechanisms that induce the circadian variation in the D2 expression, we examined the effects of glucocorticoid on the D2 activity and mRNA in rat aorta media and cultured vascular smooth muscle cells (VSMCs).METHODS:The effects of intrinsic and extrinsic glucocorticoid on the D2 activity and mRNA in rat aorta media were studied using metyrapone, a corticosterone synthesis inhibitor, and dexamethasone (DEX). Further, the effects of DEX on D2 expression were studied using the cultured rat VSMCs.RESULTS:The trough values of D2 activity and mRNA at 2100 hour were increased by the treatment with metyrapone. On the other hand, the peak values of D2 activity and mRNA were decreased by the treatment with DEX. D2 activity and mRNA in cultured rat VSMCs were increased by the addition of 10(-3) M dibutyryl cyclic adenosine monophosphate [(Bu)(2)cAMP]. The increments were reduced by coincubation with 10(-6) M DEX.CONCLUSIONS:These results suggest that glucocorticoids might directly suppress the D2 expression in rat VSMCs induced by a cAMP-dependent mechanism.
DOI 10.1089/thy.2009.0044
PMID 19508118