Department   School of Medicine, School of Medicine
   Position   Professor and Division head
Article types Original article
Language English
Peer review Peer reviewed
Title Isolation, selection and characteristics of Lactobacillus paracasei subsp. paracasei F19
Journal Formal name:Microbial Ecology in Health and Disease 
Domestic / ForeginForegin
Volume, Issue, Page 14,pp.4-6
Author and coauthor Ljungh A, Lan J, Yanagisawa N
Authorship Last author
Publication date 2002
Summary Lactobacillus strains were isolated from the deep colonic mucus layer of patients without gastrointestinal disease and screened for binding of porcine mucin. Strains exerting high mucin binding were further characterized as to survival ability in the gastrointestinal tract, viz pH stability and bile tolerance. Lactobacillus F19, identified as Lactobacillus paracasei ssp paracasei, bound gastric and bovine mucin, collagen I and III and fibronectin (Fn), and expressed high surface hydrophobicity. It survived exposure to pH 2.5 for 1 h and 20% bile for 2 h, had proteolytic activity and produced bacteriocin(s). It should thus have good possibilities to survive the passage through the gastrointestinal (GI) tract and establish in the GI mucin. Lactobacillus F19 transcribed NF- &#115 B to the nucleus of macrophages, and may thus have immunomodulatory effect.
DOI doi.org/10.1080/089106002760003279