ヤナギサワ　ナオコ   YANAGISAWA Naoko   柳沢　直子    所属   医学部 医学科    職種   教授・基幹分野長
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	The N-terminal of thrombospondin-1 is essential for coagulase-negative staphylococcal binding.
掲載誌名	正式名：Journal of medical microbiology 略　 称：J Med Microbiol ISSNコード：(0022-2615)0022-2615(Linking)
掲載区分	国外
巻・号・頁	50(8),pp.712-719
著者・共著者	Yanagisawa N, Li D Q, Ljungh A
担当区分	筆頭著者
発行年月	2001/08
概要	Bacterial binding was studied to determine whether thrombospondin-1 (TSP) acts as a ligand in attachment of coagulase-negative staphylococci (CNS). Twenty-five of 27 CNS strains bound soluble TSP. Staphylococcus epidermidis J9P bound 125I-labelled TSP in a dose-dependent manner. Scatchard plot analysis of the binding of TSP by strain J9P revealed two Kd values of 6.4 x 10(-9) M and 2.9 x 10(-8) M. The binding structures of strain J9P were sensitive to protease and were resistant to heat treatment. Unlabelled TSP and recombinant von Willebrand factor inhibited binding of TSP by strain J9P, but other proteins or monosaccharides did not. Heparin inhibited binding of TSP to strain J9P and two other S. epidermidis strains, BD5703 and BD969. Fusion proteins of the type 1 repeats, type 2 repeats, type 3 repeats and C-terminal domain of TSP or the synthetic Arg-Gly-Asp peptide did not inhibit binding of TSP to bacteria. TSP promoted adhesion of S. epidermidis strains when it was immobilised on polymer surfaces. These results indicate that the specific interaction between CNS and TSP may contribute to bacterial adhesion on biomaterial surfaces. The N-terminal heparin-binding domain of TSP appears to be the major region for recognition by CNS.
DOI	10.1099/0022-1317-50-8-712
PMID	11478675