TOKITA Daisuke
Department School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine Position |
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Article types | Original article |
Language | English |
Peer review | Peer reviewed |
Title | Liver sinusoidal endothelial cells have a capacity for inducing nonresponsiveness of T cells across major histocompatibility complex barriers. |
Journal | Formal name:Transplant international : official journal of the European Society for Organ Transplantation Abbreviation:Transpl Int ISSN code:09340874/09340874 |
Domestic / Foregin | Foregin |
Volume, Issue, Page | 18(2),pp.206-14 |
Author and coauthor | Onoe Takashi, Ohdan Hideki, Tokita Daisuke, Hara Hidetaka, Tanaka Yuka, Ishiyama Kohei, Asahara Toshimasa |
Publication date | 2005/02 |
Summary | Livers transplanted across major histocompatibility complex (MHC) barriers in mice are normally accepted without recipient immune suppression. To identify the cell type that contributes to induction of such a tolerance state, we established an allogeneic mixed hepatic constituent cell-lymphocyte reaction (MHLR) assay. Hepatic constituent cells were isolated from C57BL/6 (B6) and Balb/c mice as stimulators, and splenocytes were isolated from B6 mice as responders. Irradiated hepatic constituent cells were co-cultured with fluorescent dye (CFSE)-labeled B6 splenocytes. In the allogeneic MHLR using either whole hepatic constituent cells or parenchymal hepatocytes as stimulators, a lack of T-cell proliferation was observed. Only when CD105(+) cells, which are exclusively liver sinusoidal endothelial cells (LSECs), were depleted from hepatic constituent cell stimulators, the MHLR resulted in marked proliferation of both allo-reactive CD4(+) and CD8(+) T cells. These results indicate that CD105(+) LSECs have the capacity to induce nonresponsiveness of T cells across MHC barriers. |
DOI | 10.1111/j.1432-2277.2004.00025.x |
PMID | 15691274 |