SHIMIZU Tatsuya
Department Graduate School of Medical Science, Graduate School of Medical Science Position Professor |
|
Article types | Original article |
Language | English |
Peer review | Peer reviewed |
Title | L-Lactate treatment by photosynthetic cyanobacteria expressing heterogeneous L-lactate dehydrogenase. |
Journal | Formal name:Scientific reports Abbreviation:Sci Rep ISSN code:20452322/20452322 |
Domestic / Foregin | Foregin |
Publisher | Nature Research |
Volume, Issue, Page | 13(1),pp.7249 |
Total page number | 8 |
Author and coauthor | KATO Yuichi†, INABE Kosuke, HARAGUCHI Yuji, SHIMIZU Tatsuya, KONDO Akihiko, HASUNUMA Tomohisa* |
Publication date | 2023/05/04 |
Summary | L-Lactate is a major waste compound in cultured animal cells. To develop a sustainable animal cell culture system, we aimed to study the consumption of L-lactate using a photosynthetic microorganism. As genes involved in L-lactate utilization were not found in most cyanobacteria and microalgae, we introduced the NAD-independent L-lactate dehydrogenase gene from Escherichia coli (lldD) into Synechococcus sp. PCC 7002. The lldD-expressing strain consumed L-lactate added to basal medium. This consumption was accelerated by expression of a lactate permease gene from E. coli (lldP) and an increase in culture temperature. Intracellular levels of acetyl-CoA, citrate, 2-oxoglutarate, succinate, and malate, and extracellular levels of 2-oxoglutarate, succinate, and malate, increased during L-lactate utilization, suggesting that the metabolic flux from L-lactate was distributed toward the tricarboxylic acid cycle. This study provides a perspective on L-lactate treatment by photosynthetic microorganisms, which would increase the feasibility of animal cell culture industries. |
DOI | 10.1038/s41598-023-34289-3 |
PMID | 37142758 |