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SHIMIZU Tatsuya
Department Research Institutes and Facilities, Research Institutes and Facilities Position Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Circular cell culture for sustainable food production using recombinant lactate-assimilating cyanobacteria that supplies pyruvate and amino acids. |
| Journal | Formal name：Archives of microbiology Abbreviation：Arch Microbiol ISSN code：1432072X/03028933 |
| Domestic / Foregin | Foregin |
| Publisher | Springer nature |
| Volume, Issue, Page | 205(7),pp.266 |
| Total page number | 17 |
| Author and coauthor | HARAGUCHI Yuji†*, KATO Yuichi, INABE Kosuke, KONDO Akihiko, HASANUMA Tomohisa, SHIMIZU Tatsuya* |
| Authorship | Last author,Corresponding author |
| Publication date | 2023/06/16 |
| Summary | Recently, we reported a circular cell culture (CCC) system using microalgae and animal muscle cells for sustainable culture food production. However, lactate accumulation excreted by animal cells in the system characterized by medium reuse was a huge problem. To solve the problem, as an advanced CCC, we used a lactate-assimilating cyanobacterium Synechococcus sp. PCC 7002, using gene-recombination technology that synthesises pyruvate from lactate. We found that the cyanobacteria and animal cells mutually exchanged substances via their waste media: (i) cyanobacteria used lactate and ammonia excreted by animal muscle cells, and (ii) the animal cells used pyruvate and some amino acids excreted by the cyanobacteria. Because of this, animal muscle C2C12 cells were amplified efficiently without animal serum in cyanobacterial culture waste medium in two cycles (first cycle: 3.6-fold; second cycle: 3.9-fold/three days-cultivation) using the same reuse medium. We believe that this advanced CCC system will solve the problem of lactate accumulation in cell culture and lead to efficient cultured food production. |
| DOI | 10.1007/s00203-023-03607-8 |
| PMID | 37328623 |