SHIMIZU Tatsuya    Department   Research Institutes and Facilities, Research Institutes and Facilities    Position   Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Bioengineering of a scaffold-less three-dimensional tissue using net mould.
Journal	Formal name：Biofabrication Abbreviation：Biofabrication ISSN code：17585090/17585082
Domestic / Foregin	Foregin
Volume, Issue, Page	13(4),pp.045019
Author and coauthor	SAKAGUCHI Katsuhisa†*, TOBE Yusuke, YANG Jiayue, TANAKA Ryu-ichiro, YAMANAKA Kumiko, ONO Jiro, SHIMIZU Tatsuya
Authorship	Last author
Publication date	2021/09/17
Summary	Tissue engineering has attracted attention worldwide because of its application in regenerative medicine, drug screening, and cultured meat. Numerous biofabrication techniques for producing tissues have been developed, including various scaffold and printing methods. Here, we have proposed a novel tissue engineering method using a net metal mould without the use of a scaffold. Briefly, normal human dermal fibroblasts seeded on a dimple plate were subjected to static culture technique for several days to form spheroids. Spheroids of diameter ≥200 μm were poured into a net-shaped mould of gap ≤100 μm and subjected to shake-cultivation for several weeks, facilitating their fusion to form a three-dimensional (3D) tissue. Through this study, we successfully constructed a scaffold-free 3D tissue having strength that can be easily manipulated, which was difficult to construct using conventional tissue engineering methods. We also investigated the viability of 3D tissue and found that the condition of the tissues was completely different depending on the culture media used. Collectively, this method allows scaffold-free culture of 3D tissues of unprecedented thickness, and may contribute largely to next-generation tissue engineering products.
DOI	10.1088/1758-5090/ac23e3
PMID	34488209