SHIMIZU Tatsuya
   Department   Research Institutes and Facilities, Research Institutes and Facilities
   Position   Professor
Article types Original article
Language English
Peer review Non peer reviewed
Title Lipopolysaccharide augments expression and secretion of vascular endothelial growth factor in rat ventricular myocytes.
Journal Formal name：Biochemical and biophysical research communications
Abbreviation：Biochem Biophys Res Commun
ISSN code：(0006-291X)0006-291X(Linking)
Domestic / ForeginForegin
Publisher Elsevier
Volume, Issue, Page 268(2),pp.657-62
Author and coauthor Sugishita Y†, Shimizu T, Yao A, Kinugawa K i, Nojiri T, Harada K, Matsui H, Nagai R, Takahashi T
Publication date 2000/02
Summary Vascular endothelial growth factor (VEGF), also known as vascular permeability factor, is highly expressed in the myocardium under various stimuli including hypoxia and ischemia. On the other hand, lipopolysaccharide (LPS) causes systemic inflammatory response syndrome (SIRS), which consists of systemic pathophysiological changes related to vascular hyperpermeability. To test the hypothesis that VEGF is one of the important mediators of SIRS, we examined effects of LPS on the VEGF expression and secretion in cultured neonatal rat ventricular myocytes. LPS (10 microg/ml) rapidly (within 1 h) augmented the levels of VEGF mRNA in these cells. Pharmacological inhibition of nucleic factor-kappaB or tyrosine kinases did not affect the LPS-induced augmentation of VEGF mRNA expression, while these treatments markedly suppressed the up-regulation of inducible nitric oxide synthase (iNOS) expression by LPS. The VEGF concentrations in the conditioned media were also significantly increased by the LPS treatment of 6 h. In conclusion, LPS augments VEGF expression and secretion in rat ventricular myocytes, suggesting that VEGF may be involved in pathogenesis of SIRS. LPS may induce VEGF mRNA through the signaling pathways that are distinct from those responsible for the iNOS induction.
DOI 10.1006/bbrc.2000.2165
PMID 10679260