|
SHIMIZU Tatsuya
Department Graduate School of Medical Science, Graduate School of Medical Science Position Professor |
|
| Article types | Original article |
| Language | English |
| Peer review | Non peer reviewed |
| Title | Cloning of hypoxia-inducible factor 1alpha cDNA from chick embryonic ventricular myocytes. |
| Journal | Formal name：Biochemical and biophysical research communications Abbreviation：Biochem Biophys Res Commun ISSN code：(0006-291X)0006-291X(Linking) |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 281(4),pp.1057-62 |
| Author and coauthor | TAKAHASHI Toshiyuki†, SUGISHITA Yasuyuki, NOJIRI Takefumi, SHIMIZU Tatsuya, YAO Atsushi, KINUGAWA Koh-ichiro, HARADA Kazumasa, NAGAI Ryozo* |
| Publication date | 2001/03 |
| Summary | Hypoxia-inducible factor 1 (HIF-1) is composed of HIF-1alpha and arylhydrocarbon nuclear receptor translocator (ARNT), which belong to the basic-helix-loop-helix-Per/ARNT/Sim (bHLH-PAS) family of transcription factors. HIF plays key roles in oxygen homeostasis and embryonic cardiovascular development. In this study, we have cloned cDNAs encoding the chick HIF-1alpha from cultured embryonic ventricular myocytes (CEVM) and then examined its expression in various embryonic tissues. The deduced amino acid sequence of the chick HIF-1alpha cDNA showed 79% identity with that of the human HIF-1alpha cDNA. In contrast, sequence homology between the chick HIF-1alpha and endothelial PAS protein 1 (EPAS1), another member of the bHLH-PAS proteins, was only low (49%). HIF-1alpha mRNA was expressed abundantly in CEVM, but scarcely in the liver, which was quite different from expression pattern of EPAS1 mRNA. These data suggest that HIF-1alpha may be involved in embryonic cardiovascular development. HIF-1alpha and EPAS1 may play distinct roles during developmental stages. |
| DOI | 10.1006/bbrc.2001.4463 |
| PMID | 11237772 |