シミズ タツヤ   SHIMIZU Tatsuya
  清水 達也
   所属   研究施設 研究施設
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 A new method for manufacturing cardiac cell sheets using fibrin-coated dishes and its electrophysiological studies by optical mapping.
掲載誌名 正式名:Artificial organs
略  称:Artif Organs
ISSNコード:(0160-564X)0160-564X(Linking)
掲載区分国外
巻・号・頁 29(2),95-103頁
著者・共著者 ITABASHI Yuji†, MIYOSHI Shunichiro, KAWAGUCHI Haruko, YUASA Shinsuke, TANIMOTO Kojiro, FURUTA Akira, SHIMIZU Tatsuya, OKANO Teruo, FUKUDA Keiichi, OGAWA Satoshi*
発行年月 2005/02
概要 We developed a novel simple method for making functional myocardial cell sheets that may be used as transplants. Polymerized human fibrin-coated dishes were prepared with fibrinogen monomers mixed with thrombin. Neonatal rat cardiomyocytes cultured on these dishes formed myocardial cell sheets within 4 days. These cell sheets were easily dissociated intact from the polymerized fibrin layer, because the fibrin had been digested by intrinsic protease. Two overlaid myocardial cell sheets exhibited synchronized spontaneous beating and captured artificial pacing. Optical mapping confirmed that the conduction of the action potential between two partially overlaid myocardial cell sheets was established, and the action potential propagated across the junction without any delay. Transplanted three-layered myocardial cell sheets exhibited strong spontaneous beating and showed well-differentiated striations and an increase in cell size. This simple method of cell sheet engineering may also be applicable for various other cell types.
DOI 10.1111/j.1525-1594.2005.29020.x
PMID 15670278