シミズ タツヤ   SHIMIZU Tatsuya
  清水 達也
   所属   研究施設 研究施設
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Bioengineered cardiac cell sheet grafts have intrinsic angiogenic potential.
掲載誌名 正式名:Biochemical and biophysical research communications
略  称:Biochem Biophys Res Commun
ISSNコード:(0006-291X)0006-291X(Linking)
掲載区分国外
出版社 Elsevier
巻・号・頁 341(2),573-82頁
著者・共著者 Sekiya Sachiko†, Shimizu Tatsuya, Yamato Masayuki, Kikuchi Akihiko, Okano Teruo*
発行年月 2006/03
概要 Previously, we have demonstrated the long-term survival of myocardial cell sheet constructs in vivo, with microvascular network formation throughout the engineered tissues. The understanding and control of these vascularization processes are a key factor for creating thicker functional tissues. Here, we show that cardiac cell sheets express angiogenesis-related genes and form endothelial cell networks in culture. After non-invasive harvest and stacking of cell sheets using temperature-responsive culture dishes, these endothelial cell networks are maintained and result in neovascularization upon in vivo transplantation. Interestingly, we also discovered that all of the graft vessels are derived from the grafts themselves and these vessels migrate to connect with the host vasculature. Finally, blood vessel formation within the grafts can be controlled by changing the ratio of endothelial cells. In conclusion, myocardial tissue grafts engineered with cell sheet technology have their own inherent potential for the in vivo neovascularization that can be regulated in vitro.
DOI 10.1016/j.bbrc.2005.12.217
PMID 16434025