SHIMIZU Tatsuya    Department   Research Institutes and Facilities, Research Institutes and Facilities    Position   Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Novel cardiac precursor-like cells from human menstrual blood-derived mesenchymal cells.
Journal	Formal name：Stem cells (Dayton, Ohio) Abbreviation：Stem Cells ISSN code：(1549-4918)1066-5099(Linking)
Domestic / Foregin	Foregin
Publisher	wiley
Volume, Issue, Page	26(7),pp.1695-704
Author and coauthor	HIDA Naoko†, NISHIYAMA Nobuhiro, MIYOSHI Shunichieo, KIRA Shinichiro, SEGAWA Kaoru, UYAMA Taro, MORI Taisuke, MIYADO Kenji, IKEGAI Yukinori, CUI ChangHao, KIYONO Tohru, KYO Satoru, SHIMIZU Tatsuya, OKANO Teruo, SAKAMOTO Michie, OGAWA Satoshi, UMEZAWA Akhihiro*
Publication date	2008/07
Summary	Stem cell therapy can help repair damaged heart tissue. Yet many of the suitable cells currently identified for human use are difficult to obtain and involve invasive procedures. In our search for novel stem cells with a higher cardiomyogenic potential than those available from bone marrow, we discovered that potent cardiac precursor-like cells can be harvested from human menstrual blood. This represents a new, noninvasive, and potent source of cardiac stem cell therapeutic material. We demonstrate that menstrual blood-derived mesenchymal cells (MMCs) began beating spontaneously after induction, exhibiting cardiomyocyte-specific action potentials. Cardiac troponin-I-positive cardiomyocytes accounted for 27%-32% of the MMCs in vitro. The MMCs proliferated, on average, 28 generations without affecting cardiomyogenic transdifferentiation ability, and expressed mRNA of GATA-4 before cardiomyogenic induction. Hypothesizing that the majority of cardiomyogenic cells in MMCs originated from detached uterine endometrial glands, we established monoclonal endometrial gland-derived mesenchymal cells (EMCs), 76%-97% of which transdifferentiated into cardiac cells in vitro. Both EMCs and MMCs were positive for CD29, CD105 and negative for CD34, CD45. EMCs engrafted onto a recipient's heart using a novel 3-dimensional EMC cell sheet manipulation transdifferentiated into cardiac tissue layer in vivo. Transplanted MMCs also significantly restored impaired cardiac function, decreasing the myocardial infarction (MI) area in the nude rat model, with tissue of MMC-derived cardiomyocytes observed in the MI area in vivo. Thus, MMCs appear to be a potential novel, easily accessible source of material for cardiac stem cell-based therapy.
DOI	10.1634/stemcells.2007-0826
PMID	18420831