SHIMIZU Tatsuya
   Department   Research Institutes and Facilities, Research Institutes and Facilities
   Position   Professor
Article types Original article
Language English
Peer review Peer reviewed
Title Enhanced Therapeutic Effects of Human iPS Cell Derived-Cardiomyocyte by Combined Cell-Sheets with Omental Flap Technique in Porcine Ischemic Cardiomyopathy Model.
Journal Formal name：Scientific reports
Abbreviation：Sci Rep
ISSN code：(2045-2322)2045-2322(Linking)
Domestic / ForeginForegin
Volume, Issue, Page 7(1),pp.8824
Author and coauthor KAWAMURA Masashi†, MIYAGAWA Shigeru, FUKUSHIMA Satsuki, SAITO Atsuhiro, MIKI Kenji, FUNAKOSHI Shunsuke, YOSHIDA Yoshinori, YAMANAKA Shinya, SHIMIZU Tatsuya, OKANO Teruo, DAIMON Takashi, TODA Koichi, SAWA Yoshiki*
Publication date 2017/08
Summary Transplant of human induced pluripotent stem cell derived cardiomyocytes (hiPS-CMs) cell-sheet is a promising approach for treating ischemic cardiomyopathy (ICM). However, poor blood supply to the transplanted cell-sheet is a concern related to the effectiveness and durability of the treatment. Herein, we hypothesized that the combined the omentum flap might enhance survival and the therapeutic effects of hiPS-CM cell-sheet transplant for ICM treatment. Treatment by Wnt signaling molecules in hiPS cells produced hiPS-CMs, which were magnetically labeled by superparamagnetic iron oxide (SPIO), followed by culture in the thermoresponsive dishes to generate hiPS-CMs cell-sheets. A porcine ICM model included 4 groups; sham operation, omentum flap only, cell-sheet only, or combination therapy. Ejection fraction (EF) was significantly greater in the cell-sheet only and combination group compared to the other groups during the follow-up period. At 3 months, the EF of the combination group was significantly greater than that of the cell-sheet only group. Consistently, the survival rate of the SPIO-labeled hiPS-CMs, as assessed by MRI, was significantly greater in the combination group than in the cell-sheet only group. This cell delivery system would be useful in optimizing the hiPS-CM cell-sheet transplant for treating severe heart failure.
DOI 10.1038/s41598-017-08869-z
PMID 28821761