シミズ タツヤ   SHIMIZU Tatsuya
  清水 達也
   所属   研究施設 研究施設
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Enhanced Therapeutic Effects of Human iPS Cell Derived-Cardiomyocyte by Combined Cell-Sheets with Omental Flap Technique in Porcine Ischemic Cardiomyopathy Model.
掲載誌名 正式名:Scientific reports
略  称:Sci Rep
ISSNコード:(2045-2322)2045-2322(Linking)
掲載区分国外
巻・号・頁 7(1),8824頁
著者・共著者 KAWAMURA Masashi†, MIYAGAWA Shigeru, FUKUSHIMA Satsuki, SAITO Atsuhiro, MIKI Kenji, FUNAKOSHI Shunsuke, YOSHIDA Yoshinori, YAMANAKA Shinya, SHIMIZU Tatsuya, OKANO Teruo, DAIMON Takashi, TODA Koichi, SAWA Yoshiki*
発行年月 2017/08
概要 Transplant of human induced pluripotent stem cell derived cardiomyocytes (hiPS-CMs) cell-sheet is a promising approach for treating ischemic cardiomyopathy (ICM). However, poor blood supply to the transplanted cell-sheet is a concern related to the effectiveness and durability of the treatment. Herein, we hypothesized that the combined the omentum flap might enhance survival and the therapeutic effects of hiPS-CM cell-sheet transplant for ICM treatment. Treatment by Wnt signaling molecules in hiPS cells produced hiPS-CMs, which were magnetically labeled by superparamagnetic iron oxide (SPIO), followed by culture in the thermoresponsive dishes to generate hiPS-CMs cell-sheets. A porcine ICM model included 4 groups; sham operation, omentum flap only, cell-sheet only, or combination therapy. Ejection fraction (EF) was significantly greater in the cell-sheet only and combination group compared to the other groups during the follow-up period. At 3 months, the EF of the combination group was significantly greater than that of the cell-sheet only group. Consistently, the survival rate of the SPIO-labeled hiPS-CMs, as assessed by MRI, was significantly greater in the combination group than in the cell-sheet only group. This cell delivery system would be useful in optimizing the hiPS-CM cell-sheet transplant for treating severe heart failure.
DOI 10.1038/s41598-017-08869-z
PMID 28821761